NGS Workflow Steps - Next-Generation Sequencing

Unlocking The Most Cost-Effective & Reproducible NGS Workflow

Although reaction miniaturization is ubiquitous in large pharmaceutical environments, it can be challenging to understand the risks and considerations associated with generating high-quality data in the context of miniaturizing assays.  

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The DISPENDIX team has worked through miniaturization of even the most complex NGS workflows, and can help you to scale volumes as low as 1/10th the manufacturers recommended SOP without compromising data quality.

Normalization & Pooling

Following library preparation, quality control analysis provides the concentration of each library individual NGS library. In the case where samples will be pooled for cost-effective sequencing, they must be normalized to the same concentration to avoid oversampling of one library relative to another.

If you want to learn more, please visit our website TSKT.

The I.DOT can perform a normalization of up to 96 NGS libraries in minutes with a 1 μL dead volume per sample, providing a much more efficient solution than manual pipetting and traditional automated liquid handlers.

Further, the I.DOT can pool a variable volume of each sample into a single well allowing for normalization and pooling to occur in a single step - now that is efficiency!

Are you interested in learning more about NGS Library Prep Kits? Contact us today to secure an expert consultation!